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Cell Analysis Section

Education, tools

• FRET (Förster or fluorescence resonance energy transfer)

  Contributed by Péter Nagy

  • FRET tutorial (PDF file, YouTube video)
  • Anisotropy and FRET (Flash animation)

• Evaluation of FRET experiments

  • evaluation of intensity-based (ratiometric) microscopic FRET measurements: rFRET in Matlab
  • evaluation of intensity-based (ratiometric) microscopic FRET measurements: RiFRET, an ImageJ plugin
  • evaluation of intensity-based (ratiometric) flow cytometric FRET measurements: ReFLEX, a Windows program
  • acceptor photobleaching: AccPbFRET, in ImageJ plugin
  • evaluation of intensity-based (ratiometric) FRET measurements carried out using a laser scanning cytometer (CellProfiler pipeline)

• Flow cytometry

  Contributed by Péter Nagy, in Hungarian

  • Flow cytometry tutorial (PDF file, YouTube video)
  • Signal detection in flow cytometry (Flash animation)

• "Number and brightness" (N&B) and colocalization analysis

  Contributed by Péter Nagy, in Hungarian

  • “Number and brightness” (N&B) and colocalization analysis (PDF)

• Electron microscopy

  Contributed by Péter Nagy

  • Electron microscopy (PDF)

• Digital image analysis

  Contributed by Péter Nagy

  Digital image analysis (PDF)

• Fluorescence correlation spectroscopy

  Contributed by György Vámosi

  • Fluorescence correlation spectroscopy (PDF)