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Szabó Bálint
Automated single cell isolation on a microscope

Aug 29 - kedd

15:30 – 17:00

I. Poszterszekció

P28

Automated single cell isolation on a microscope

Bálint Szabó

CellSorter Kft.

Current robots for single cell manipulations can only handle surface attached cells limiting the field of applications. CellSorter developed a computer vision-based robot to automatically recognize and gently isolate intact individual cells (for subsequent analysis, e.g., DNA/RNA sequencing) from suspension without immobilizing cells on the surface of the Petri dish. We used a motorized microscope and robotic micropipette controlled by the computer vision of cells. Combination of 1 µm positioning precision, adaptive cell targeting and < 1 nl liquid handling precision resulted in an unprecedented accuracy in robotic cell isolation. Single cells could be picked up in a volume of 1.4 ±0.6 nl without removing neighboring cells. Sorting process did not affect the viability of cells. We compared the efficiency of our method to that of single cell entrapment in microwells and subsequent sorting with the automated micropipette: the recovery rate of single cells was greatly improved. Our straightforward technique needs minimal sample preparation and can be applied for virtually any tissue cell type. We expect new applications in circulating tumor cell (CTC) detection and isolation for molecular characterization and personalized treatment.