Összes szerző

---

Strádi Andrea

az alábbi absztraktok szerzői között szerepel:

Madas Balázs
Metastatic potential of HeLa-cells does not increase directly after radiation exposure

Aug 29 - kedd

14:20 – 14:40

Orvosi biofizika és sugárbiológia

E15

Metastatic potential of HeLa-cells does not increase directly after radiation exposure

Balázs Madas¹, Kinga Kovács², Andrea Strádi³, Szabolcs Polgár⁴, Inna Székács⁵, and Róbert Horváth⁵

¹ Centre for Energy Research, Environmental Physics Department, Budapest, Hungary

² Centre for Energy Research, Nanobiosensorics Department, Budapest, Hungary

³ Centre for Energy Research, Space Research Department, Budapest, Hungary

While radiation therapy increases local tumor control, it remains controversial whether ionizing radiation increases the metastatic potential of cancer cells. One of the potential mechanisms of radiation-induced metastasis is the direct release of tumor cells into the circulation requiring the detachment of the cells. The objective of the present study was to directly measure how ionizing radiation affects the kinetics of cellular adhesion, especially its initial stage after cell attachment on a biomimetic surface.

For this purpose, an automatic irradiation facility with gamma-radiation from Cs-137 has been developed providing parallel irradiation opportunity of 96 wells of a biosensor microplate with different doses. The employed optical biosensor records the wavelength shift of reflected light from a nanostructured waveguide, being proportional to the cell adhesion strength. Absorbed doses were measured by thermoluminescent dosimeters (TLDs) in each well. As a model system, a cervical cancer cell line (HeLa) was studied.

Three different experimental setups have been used distinguished by the sequence of irradiation and cellular attachment to the surface. The wavelength shift as the function of time was measured for different absorbed doses. The maximum wavelength shift as the function of dose was also analyzed.

The results show that adhesion of HeLa cells is not affected by ionizing radiation in the first hours after irradiation. The result is independent of whether cells are exposed during the adhesion process, in suspension, or attached. This suggests that radiation therapy does not directly increase the metastatic potential of cancer cells by decreasing their adhesion.

The experimental setup can be used to quantify the effects of ionizing radiation on cell adhesion as the function of time at different absorbed doses. It has been shown that ionizing radiation does not affect the adhesion of HeLa cells in the first hours after exposure, while experiments with longer follow-up are required to see whether adhesion changes at later time points.