Összes szerző
Steinbach Gábor
az alábbi absztraktok szerzői között szerepel:
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Steinbach Gábor
Anisotropy imaging using RCM -
Aug 29 - kedd
15:30 – 17:00
I. Poszterszekció
P26
Anisotropy imaging using RCM
Gábor Steinbach1,2, Dávid Nagy3, Gábor Sipka2,4 , Ábel Garab2 , Győző Garab2,4 and László Zimányi3
1 ELKH, Biological Research Centre, Szeged, C ellular Imaging Laboratory
2 Biofotonika R&D Ltd.
3 ELKH, Biological Research Centre, Szeged, Institute of Biophysics
4 ELKH, Biological Research Centre, Szeged, Institute of Plant Biology
Both the differential polarization attachments for LSMs and Re-scan Confocal Microscopy (RCM) facilitate revealing structures at the molecular level [1]. For subcellular structures, increasing the available resolution can be fundamental. The re-scan confocal microscopy (RCM) provides 4 times better signal to noise ratio (compared to conventional PMTs) using an sCMOS camera, moreover, due to the second scanner, the image resolution increases by the factor of 1.4, while the axial resolution is identical with the LSMs [2].
With additional polarization elements, the RCM enables the 2D and 3D microscopic mapping of the anisotropy of samples via measuring fluorescence-detected linear dichroism (FDLD). The usage of the liquid crystal modulator for the RCM and the high frequency modulation (photoelastic modulator – PEM) are synchronised with the imaging. The DP-LSMs and the pRCM (RCM equipped with polarization attachment) are suitable for obtaining unique structural information on the anisotropic molecular organization of biological samples and intelligent materials [3, 4] in 2D and 3D.
References
[1] Steinbach et al. (2019) European Biophysics Journal 48: 457-463., doi: 10.1007/s00249-019-01365-4
[2] De Luca GMR et al. (2017) Methods Appl Fluoresc 5:015002., doi: 10.1088/2050-6120/5/1/015002
[3] Simonović Radosavljević J et al. (2021) Int. J. Mol. Sci. 22: 7661, doi: 10.3390/ijms22147661
[4] Pleckaitis M et al. (2022) Nano Research, 15: 5527-5537., doi: 10.1007/s12274-021-4048-x