Összes szerző

Quemé-Peña Mayra

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Juhász Tünde
Co-assemblies of cationic antimicrobial peptides with anionic small molecules: unique thermophoretic behaviour

Aug 29 - kedd

15:30 – 17:00

I. Poszterszekció

P15

Co-assemblies of cationic antimicrobial peptides with anionic small molecules: unique thermophoretic behaviour

Tünde Juhász1, Mayra Quemé-Peña1, Tamás Beke-Somfai1

1 Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences

Peptidic supramolecules formed via self- and co-assembly show emerging applications from materials chemistry to biomedicine. Recently we have demonstrated for a couple of cationic, amphiphilic antimicrobial peptides (AMPs) the ability to form co-assemblies with various anionic, aromatic small molecule (SM) binding agents. Their interaction results in modulating their activity in vitro. Herein we addressed interaction systems of four selected AMPs (CM15, Dhvar4, LL-37, and FK-16, coupled to carboxyfluorescein) with four selected SMs (suramin, tartrazine, biliverdin, and bilirubin ditaurate) exploiting the sensitivity of microscale thermophoresis (MST). MST is a solution phase method utilizing the phenomenon of induced thermophoresis where movement of the molecules is monitored via the fluorescence of the target partner. Besides substantial similarities, various scenarios of thermophoretic responses were revealed. Interestingly, negative thermophoresis was frequently observed, and maximal MST responses were typically found not at the highest SM concentrations applied. Findings suggest substantial contributions of charge and hydration effects. Variations in the thermophoretic behaviour could also be attributed to the oligomeric state of the individual components, and the dynamic nature of the association process. Results demonstrate that the MST method is an excellent additional technique for identifying and studying peptidic assemblies.

Acknowledgment

The authors thank for support from LP2016-2, NVKP_16-1-2016-0007, TKP2021-EGA-31, KKP22 144180, SA-87/2021, KEP-5/2021, and 2020-1-1-2-PIACI-KFI_2020-00021.