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Mészáros Ádám

az alábbi absztraktok szerzői között szerepel:

Dudás Tamás
Capillary pericytes regulate vascular tone and local blood flow in inflammation

Aug 29 - kedd

15:00 – 15:15

Orvosi biofizika és sugárbiológia

E17

Capillary pericytes regulate vascular tone and local blood flow in inflammation

Tamás Dudás, Ádám Mészáros, Kinga Molnár, Attila Farkas, Imola Wilhelm and István Krizbai

Biological Research Centre, Szeged, Institute of Biophysics

Pericytes are the only contractile cells in cerebral capillaries. However, their role in the regulation of capillary diameter, microvascular tone and local cerebral blood flow is far from being completely understood. Furthermore, a large number of CNS disorders is accompanied by inflammatory processes. Therefore, in our present study, we investigated the role of pericytes in the maintenance of capillary tone and how inflammatory mediators could regulate pericyte contractility.

Using primary human pericytes in an in vitro collagen contraction assay, we could demonstrate that TNF-alpha, IL-6 and CCL2 induce a significant pericyte contraction. In order to prove that inflammatory mediators have similar effects in vivo, we used two photon microscopy in mice with labelled pericytes. Inflammatory mediators were administered in the vicinity of identified pericytes using microinjection techniques under continuous monitoring. TNF-alpha induced a slow but significant reduction in the capillary diameter. In addition, using line scan technology, we could show a decrease in red blood cell velocity and a reduction in the number of red blood cells passing the capillary segment in the neighbourhood of the injection. Furthermore, careful ablation of pericytes using the two-photon laser led to a late onset (after 24 hours) dilation of the capillary segment belonging to the ablated pericyte.

Our results indicate that pericytes may have an important role in the maintenance of the capillary tone, and may regulate capillary flow under inflammatory conditions.