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Magyaródi Beatrix

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Magyaródi Beatrix
Role of glycocalyx in cancer cell adhesion: kinetics of interactions from label-free optical biosensor measurements

Aug 30 - szerda

15:30 – 17:00

II. Poszterszekció

P46

Role of glycocalyx in cancer cell adhesion: kinetics of interactions from label-free optical biosensor measurements

Beatrix Magyaródi¹, Boglárka Kovács¹, Inna Székács¹, Robert Horvath¹

¹ Nanobiosensorics Laboratory, Research Centre for Energy Research, Institute for Technical Physics and Materials Science, Konkoly-Thege u 29-33, 1120 Budapest, Hungary

The glycocalyx is a sugar rich layer covering the surface of the cells.[1] It is composed of glycoproteins and proteoglycans. The cellular glycocalyx plays an important, but not yet understood, role in cellular signaling and metabolism, its disorders generate pathological process.[2] Interestingly, the thickness of the glycocalyx layer of cancer cells is significantly larger compared to that of healthy cells. This fact further highlights the importance of glycocalyx in tumor progression and treatment. In an earlier work, a regulatory mechanism of cellular glycocalyx in cancer adhesion was revealed using label-free optical biosensor, fluorescent microscopy, and cell surface charge measurements. [3]

The primary goal of our work is to study the role of glycocalyx components in cellular adhesion by employing various types of digesting methods. In these initial measurements we use a label-free, high-throughput, resonant waveguide grating-based optical biosensor. The instrument is well suited for monitoring of cellular adhesion kinetics in real-time, even at the single-cell level.[4]

Acknowledgment

This work was supported by the National Research, Development, and Innovation Office (Grant Numbers: PD 134195 for Z.Sz, PD 131543 for B.P., ELKH topic-fund, "Élvonal" KKP_19 TKP2022-EGA-04 grants)

References

[1] M. J. Paszek et al., “The cancer glycocalyx mechanically primes integrin-mediated growth and survival,” Nature, vol. 511, no. 7509, pp. 319–325, 2014, doi: 10.1038/nature13535.

[2] E. R. Cruz-Chu, A. Malafeev, T. Pajarskas, I. V. Pivkin, and P. Koumoutsakos, “Structure and response to flow of the glycocalyx layer,” Biophys. J., 2014, doi: 10.1016/j.bpj.2013.09.060.

[3] N. Kanyo et al., “Glycocalyx regulates the strength and kinetics of cancer cell adhesion revealed by biophysical models based on high resolution label-free optical data,” Sci. Rep., 2020, doi: 10.1038/s41598-020-80033-6.

[4] M. Sztilkovics et al., “Single-cell adhesion force kinetics of cell populations from combined label-free optical biosensor and robotic fluidic force microscopy,” Sci. Rep., 2020, doi: 10.1038/s41598-019-56898-7.