Összes szerző

Krasznai Zoárd

az alábbi absztraktok szerzői között szerepel:

Jusztus Vivien
Ion channel expression of CD8+ T cells in ovarian cancer

Aug 30 - szerda

15:30 – 17:00

II. Poszterszekció

P43

Ion channel expression of CD8⁺ T cells in ovarian cancer

Vivien Jusztus¹, Ghofrane Medyouni¹, Orsolya Vörös¹, Zsolt Szabó¹, Rudolf Lampé³, György Panyi¹, Orsolya Matolay³, Eszter Maka³, Zoárd Krasznai³, Péter Hajdu^1,2

¹ University of Debrecen, Faculty of Medicine, Department of Biophysics and Cell Biology

² University of Debrecen, Faculty of Dentistry, Division of Dental Biochemistry

³ University of Debrecen, Faculty of Medicine, Department of Gynecology and Obstetrics

The ion channels of T lymphocytes have an important role in effector functions such as activation, cytokine production and tumor cell elimination. T cells recognise and kill cancer cells during continuous monitoring. Although tumor infiltrating lymphocytes (TILs) are able to penetrate the tumor, they cannot fulfil their effector function due to the suppressive nature of the tumour microenvironment. K⁺ channels, such as Kv1.3 and KCa3.1, stabilize the negative membrane potential of T cells to control Ca²⁺-influx through CRAC channels and Ca²⁺-dependent signaling. In the present study, we determined the expression of T cell ion channels from peripheral blood of untreated ovarian cancer patients and healthy donors.

PBMCs were isolated from blood of ovarian patients and healthy donors using Ficoll-Paque density gradient method. Cells were activated with CD3/CD28 antibodies. Whole-cell current was measured in activated CD8⁺ T cells using patch-clamp technique. Ca²⁺ response of CD8⁺ cells was evaluated with FURA-2 Ca²⁺-imaging method.

KCa3.1 expression level in blood CD8⁺ cells from malignant tumor patients were lower than in healthy and benign tumor groups. Contrary, the Kv1.3 conductance of CD8⁺ T cells were significantly higher in malignant tumor patients as compared to other two groups. To asses Ca²⁺ response of cells, we determined the quotient of FURA-2 ratios measured in 2 mM Ca²⁺ and 0 mM Ca²⁺ after thapsigargin addition: there was no differences between the groups.

In summary, we suppose that down-regulation of KCa3.1 expression and Kv1.3 level upregulation in blood CD8⁺ cells could be a reporter on the presence of malignancy, as we reported before for head and neck cancer patients [1].

Acknowledgment

This work was supported by Stipendium Hungaricum Scholarship to M.G. and NKFIH (K128525, P.H.).

References

[1] Chimote AA, Balajthy A, Arnold MJ, Newton HS, Hajdu P, Qualtieri J, et al. (2018) Sci Signal. 11(527): 1–12