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Ujfalusi Zoltán
The effects of contrast agents on renal cell lines and on the actin cytoskeleton

Aug 30 - szerda

15:30 – 17:00

II. Poszterszekció

P33

The effects of contrast agents on renal cell lines and on the actin cytoskeleton

Szilvia Barkó1, Elek Telek1, Kinga Ujfalusi-Pozsonyi1 Gábor Hild1,2 and Zoltán Ujfalusi1

1 Department of Biophysics, Medical School, University of Pécs; Pécs, Hungary

2 Department of Medical Imaging, Clinical Centre, University of Pécs, Pécs, Hungary

Medical images may show some degree of contrast loss in most imaging techniques. In such cases contrast materials are the best tools to enhance the density and intensity of the given area. Nowadays, radiologists can choose from a wide range of contrast agents. The active material of these contrast media penetrates in cells and because of their limited ability of depletion these molecules can accumulate in cells of different tissues. In many cases, contrast agents are used in relatively high volumes, which places a heavy burden on kidney function. A few years ago, contrast agent treatment was identified as the third leading cause of hospital-acquired acute kidney injury (after surgery and hypotension), accounting for 12% of all cases. Today, ~5% of hospitalized patients who develop acute renal failure have normal renal function before contrast administration. We believe that contrast agents exert a significant proportion of their cell-damaging effects by affecting the actin cytoskeleton. The overview of the corresponding literature clarifies that the effects of the clinically applied contrast materials expressed directly on the actin cytoskeleton and its detailed molecular mechanisms are unknown. Our aim is to investigate all possible effects contrast materials can express on human renal cells and the actin protein inside, especially the dynamic organization/rearrangement of the actin network.

Our results clearly show that the applied contrast materials greatly affect the polymerization properties of actin. The examined contrast compounds changed other parameters of actin too and caused dramatic changes on the examined cell lines as well. The DSC results show significantly decreased thermal stability for the treated actin filaments.