Összes szerző

Bóta Attila

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Bóta Attila
Nanoerythrosome-based promising drug delivery system

Aug 30 - szerda

15:30 – 17:00

II. Poszterszekció

P38

Nanoerythrosome-based promising drug delivery systems

Attila Bóta¹, Judith Mihály¹, Kinga Ilyés¹, Bence Fehér², Tünde Juhász³, András Wacha¹, Heinz Amenitsch⁴ and Zoltán Varga¹

¹Research Centre for Natural Sciences, Biological Nanochemistry Research Group, Budapest

²Laboratory of Self-Organizing Soft Matter, Department of Chemical Engineering and Chemistry, Eindhoven University of Technology, Eindhoven, The Netherlands

³Research Centre for Natural Sciences, Biomolecular Self-assembly Research Group, Budapest

⁴Austrian SAXS beamline@ELETTRA, Are Science Park, Basovizza TS, Trieste, Italy and Inorganic Chemistry, Graz University of Technology, Graz, Austria

Nanoerythrosomes are artificial vesicle-like objects formed from erythrocyte-membranes, named ghosts, by physical processes, such as extrusion or sonication. Phosphatidylcholines (PCs) and sphingomyelins (SMs) are outer membrane constituents, while phosphatidylserines (PSs) and phosphatidylethanolamine (PEs) generally take place on the inner side of the membrane-bilayer. By addition of different artificial lipids, very different size-ranges of nanoerythrosomes can be achieved, therefore proper reference materials and drug delivery systems with adequate surface chemical behaviour can be prepared [1]. The presence of dipalmitoyl-phosphatidylethanolamine (DPPE) results in the formation of larger nanoerythrosomes, while the addition of dipalmitoylphosphatidylcholine (DPPC) induces the formation in a middle-range (140 -160 nm). The presence of the mixture of DPPC - LPC (lysophosphatidylcholine) causes bicelle – micelle type nanoparticles [2]. Here we show that in the complex physic-chemical study, among the different experimental methods (transmission electron-microscopy combined with freeze-fracture (FF-TEM), Microfluidic Resistive Pulse Sensing (MRPS), dynamic light scattering (DLS), the small-angle X-ray scattering (SAXS) turned out to be a powerful tool in the complex physic-chemical study of this drug delivery system.

Acknowledgment

The project was supported by the National Research, Development and Innovation Office of Hungary under grants K131657 (A. Bóta) and K131594 (J. Mihály) and 2018-1.2.1-NKP-2018-00005 under the 2018-1.2.1-NKP funding scheme (A. Bóta, Z. Varga). Z Varga and A. Wacha are supported by the János Bolyai Research Scholarship of the HAS.

References

[1] Deák R, Mihály J, Szigyártó ICS, Beke-Somfai T, Turiák L, Drahos L, Wacha A, Bóta A and Varga Z (2020)  Mat. Sci. and Eng. C 109:110428-110437.

[2] Bóta A, Fehér B, Wacha A, Juhász T, Szabó D, Turiák L, Gaál A, Varga Z, Amenitsch H and Mihály J(2023) J Mol. Liq. 369: 120791-120800.

Farkasné Bebesi Tímea
Spectroscopic study of extracellular vesicles using plasmonic gold nanoparticles

Aug 30 - szerda

15:30 – 17:00

II. Poszterszekció

P36

Spectroscopic study of extracellular vesicles using plasmonic gold nanoparticles

Tímea Bebesi Farkasné^1,2, Marcell Pálmai¹, Imola Csilla Szigyártó¹, Anikó Gaál¹, Orsolya Bálint-Hakkel³, Attila Bóta¹, Zoltán Varga¹, Judith Mihály¹

¹ Research Centre for Natural Sciences, Institute if Material and Environmental Sciences

² Eötvös Lóránd University, Hevesy György PhD School of Chemistry

³ Centre for Energy Research, Institute of Technical Physics and Material Sciences

Extracellular vesicles (EVs), spontaneously released by cells, play an important role in intercellular communication. Due to their special size and composition (lipid bilayer-bounded nanosystems, usually smaller than 200 nm, containing both proteins and RNA), they play diagnostic, prognostic and therapeutic roles, for example, they can be "new generation" biomarkers of various diseases.

IR spectroscopy, especially attenuated total reflection (ATR), is rapidly emerging as a label-free promising tool for molecular profiling of EVs. However, the relative low number of extracellular vesicles (~10¹⁰ particle/mL) and possible impurities (protein aggregates, lipoproteins, buffer molecules, etc.) present in EV samples might result in poor signal-to-noise (S/N) ratio. The plasmonic properties of gold nanoparticles (AuNPs) are used in many characterization techniques, inclusive characterization and testing of EVs. Surface-enhanced infrared spectroscopy (SEIRA – Surface-enhanced IR absorption) using plasmonic nanoparticle, however, is still an unexploited method.

Nanosized gold nanoparticles and tailored nanostructures with confined electromagnetic near-fields were prepared, characterized and tested with model-EVs (EV-like liposomes) and red blood cell derived EVs. A concentration dependent interaction was established between the citrate-stabilized gold nanoparticles and the lipid bilayers, which strongly affected both the plasmonic behaviour of AuNPs and the bilayers lipid organization. At appropriate extracellular vesicle – gold nanoparticle ratio a 6-fold maximum enhancement was obtained in the lipid spectral signatures. Exploiting the fine details of EV – gold nanoparticles interaction, further surface modifications of gold nanoobjects are planned, enhancing the sensitivity and specificity of EV detection enabling a strong platform for IR spectroscopic investigations of EVs.

Acknowledgment

This work was funded by ÚNKP-22-3-II-ELTE-507 and NKFIH K-131657, K131594, 2020-1-1-2- PIACI-KFI_2020-00021, TKP2021-EGA and KKP_22-144180 grants. ZV and MP are supported by the János Bolyai Research Scholarship of the HAS.

Fehér Bence
Modelling of small-angle scattering data on the absolute scale: investigation of model system NaPSS/DTAB and potentials for structural biophysics applications

Aug 29 - kedd

12:00 – 12:15

Modern biofizikai módszerek

E12

Modelling of small-angle scattering data on the absolute scale: investigation of model system NaPSS / DTAB and potentials for structural biophysics applications

Bence Fehér^1,2, Attila Bóta³ , András Wacha³, Bálint Jezsó³, Jan Skov Pedersen² and Imre Varga^4,5

¹ Laboratory of Self-Organizing Soft Matter, Department of Chemical Engineering and Chemistry, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands

² Department of Chemistry and Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark

³ Research Centre for Natural Sciences, Institute of Materials and Environmental Chemistry, Magyar tudósok körútja 2, 1117 Budapest, Hungary

⁴ Institute of Chemistry, Eötvös Loránd University, Pázmány Péter sétány 1/A, 1117 Budapest, Hungary5

⁴ Department of Chemistry, University J. Selyeho, 945 01 Komárno, Slovakia

Polyelectrolyte / surfactant (P/S) complexes are important model systems of biologically relevant complexes such as DNA, RNA, protein / surfactant complexes. Due to the structural complexity of biologically relevant polyelectrolytes they are often modelled by synthetic polyelectrolytes in order to investigate the fundamental thermodynamics of the system. There are several studies aiming at describing the structural and phase properties of P/S systems. In the phase-separation region, examples of hexagonal, lamellar, and cubic structure have been identified. At the same time, there is little information about the structure of the complexes in the one-phase region and only a few studies were published dealing with the effect of surfactant concentration on the morphology of P/S aggregates.

In our study we prepared PSS/DTAB samples in the equilibrium and phase separation range. Since in the recent decades it has been clearly shown that the high charge density P/S mixtures are prone for the formation of kinetically arrested non-equilibrium aggregates, we used a novel sample preparation method to facilitate the formation of the equilibrium non-aggregated P/S complexes in the entire investigated surfactant concentration range. We measured the binding isotherm and simultaneously we performed small-angle X-ray scattering measurements and performed data modelling with least-square fitting method on absolute scale. We showed that by increasing the binding ratio the formed complexes exhibit an elongated to spherical transition which ends with well-defined 3 dimensional hexagonal precipitate with spherical particles in the crystal points. We also showed that the addition of indifferent electrolyte (NaCl) changes the structural behavior of the system and yields amorphous structure. Summarizing, first in the literature we connected the binding isotherm to the structural features of complexes in the equilibrium and phase separation regime.

Acknowledgment

This research received funding from the Hungarian National Research, Development and Innovation Office (NKFIH K116629), which is gratefully acknowledged. This publication was also supported by the Operational Programme Research and Innovation for the project: “Support of research and development capacities in the area of nanochemical and supramolecular systems”, code ITMS2014 + 313011T583, funded from the resources of the European Regional Development Fund.