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Tóth Gabriella
Examinations of cellular uptake of cell penetrating peptides in vitro and in vivo

Aug 30 - szerda

15:30 – 17:00

II. Poszterszekció

P59

Examinations of cellular uptake of cell penetrating peptides in vitro and in vivo

Gabriella Tóth1, Gyula Batta1,2, Levente Kárpáti3, Árpád Szöőr1, István Mándity3, Péter Nagy1

1University of Debrecen, Faculty of Medicine, Department of Biophysics and Cell Biology

2University of Debrecen, Faculty of Science and Technology, Institute of Biotechnology, Department of Genetics and Applied Microbiology

3Semmelweis University, Faculty of Pharmacy, Institute of Organic Chemistry

Cell-penetrating peptides (CPPs) are peptides that enter cells by endocytosis and/or directly through the cell membrane. CPPs in general have been considered potential carriers of molecules that have difficulties entering cells. This is the feature that we would like to exploit and thereby establishing the opportunity for CPPs to have therapeutic applications in the long term. Our previously published results have shown that we can increase the cellular uptake and endosomal release of CPPs with statins. Our goal was to modify them and test if it is possible to make them enter the cells more efficiently. We also aimed to test the biodistribution of CPPs in mice after intravenous administration. We examined the cellular uptake and endosomal release by flow cytometry and confocal microscopy in SKBR-3 and MDA-MB-231 cell lines, while for the in vivo experiments a mouse model was applied. Fluorescently-labeled CPPs were used both in the in vivo and in vitro experiments. We compared the differences in the biophysical properties of the original and the modified CPPs, and we found that the cellular uptake of the modified version is more effective. There is a difference between the enhancement in the uptake of CPPs labeled by the pH-sensitive naphthofluorescein or Alexa Fluor 532. In the case of in vivo experiments, we found that peptides enter the mouse organs, including the liver, for which we have shown that CPPs is present in the intracellular space of hepatocytes. CPPs hold promise for increasing the efficiency and specificity of drug delivery to cells.