MBFT XXVII. Kongresszus - 2019, Debrecen .

Összes szerző

Rebeca Martínez Vázquez

az alábbi absztraktok szerzői között szerepel:

Kelemen Lóránd Label-free protein detection with an optofluidic lab-on-chip sensor

Aug 28 - szerda

08:30 – 08:55

Bioszenzorika és bio-nanotechnológia

E23

Label-free protein detection with an optofluidic lab-on-chip sensor

Lóránd Kelemen¹,*, Eugenia Lepera², Bence Horváth¹, Pál Ormos¹, Roberto Osellame² and Rebeca Martínez Vázquez²

¹ Biological Research Centre, Institute of Biophysics, Hungarian Academy of Sciences, Temesvári krt. 62, 6726 Szeged, Hungary;

² Institute for Photonics and Nanotechnologies, National Research Council, Piazza Leonardo da Vinci, 32, 20133 Milan, Italy

Whispering gallery mode (WGM) resonators are promising optical structures for microfluidic label-free biosensors mainly due to their high sensitivity. Their real laboratory diagnostic application however lacks a robust fabrication method that offers a complete device of practical value. Here we report on a monolithic lab on a chip sensor fabricated by a hybrid femtosecond laser micromachining approach, for label-free biosensing of a selected protein. It consists of a polymer WGM microresonator sensor made by two-photon polymerization directly inside a glass microfludic chip prepared by laser-assisted etching. The device, after a thorough geometry optimization, presents a refractive index change sensitivity of 61 nm/RIU. We demonstrate its bio-sensing capability exploiting the biotin-streptavidin binding affinity, obtaining a detectable minimum protein surface density increase of 67 x 103 molecules/µm2.

Acknowledgements

This work was supported by funding received from the CONCERT-Japan Photonic Manufacturing Joint Call (FEASIBLE project), GINOP-2.3.2-15- 2016-00001, GINOP-2.3.3-15-2016-00040 and the European Union's Horizon 2020 Research and Innovation Programme under grant agreement No. 654148 Laserlab-Europe.

Vizsnyiczai Gaszton Don’t be a fool, use a microtool: Biophotonic toolbox for single cell studies

Aug 28 - szerda

13:30 – 15:30

II. Poszterszekció

P52

Don’t be a fool, use a microtool: Biophotonic toolbox for single cell studies

Gaszton Vizsnyiczai¹, Tamás Fekete¹, Mária Mészáros¹, András Búzás¹, Gergely Iványi¹, Ádám Apró¹, Pál Ormos¹, Rebeca Martínez Vázquez² and Lóránd Kelemen¹

¹ Biological Research Centre, Institute of Biophysics, Hungarian Academy of Sciences, Temesvári krt. 62, 6726 Szeged, Hungary

² Institute for Photonics and Nanotechnologies, National Research Council, Piazza Leonardo da Vinci, 32, 20133 Milan, Italy

Microfluidics has revolutionized biological research due to its capabilities that enables measurements in extremely small volumes in a highly parallelized and integrated manner, for a relatively low cost. Considering cell studies in a microfluidic system, engineered microenvironments can add extra possibilities to the experiments: besides the plain walls, geometrical or chemical modifications, or even microdevices can be created inside the microfluidic channels. Here we introduce an assortment of microstructures and tools we have created, tested and offer for single cell studies in microchannels. These include channel-attached as well as mobile devices which can be actuated with optical tweezers, as follows.

We fabricated microslits with sub-micrometer opening to study cancer cell migration through confined spaces.

An integrated whispering-gallery mode detector was made inside a microchannel and the presence of protein was detected with it in micromolar concentration in a selective way.

The overall deformability of a single cell can be measured with a two-arm micro lever that can multiply the force the optical tweezers exerts.

Local cell deformability and its Young-modulus were obtained with an optically actuated microtool that can make small indentation on the cell membrane.

A similar tool with functionalized surface is used to test adhesion forces of various ligands to their corresponding membrane-bound receptors.

Multiview microscopic imaging of single cell was performed using microtools that are attached to the cells enabling their indirect optical manipulation; in cases when attachment is not possible, two manipulators can be used to grab and move a cell.

We plan to extend our fluorescent microscopic system which is outfitted with the optical trap towards light sheet excitation to improve image equality and data collection speed. One of our planned biological applications is to study single cell interactions between various fungi species and immune cells.

Acknowledgements