Összes szerző
Horváth Bence
az alábbi absztraktok szerzői között szerepel:
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Kelemen Lóránd
Label-free protein detection with an optofluidic lab-on-chip sensor -
Aug 28 - szerda
08:30 – 08:55
Bioszenzorika és bio-nanotechnológia
E23
Label-free protein detection with an optofluidic lab-on-chip sensor
Lóránd Kelemen1,*, Eugenia Lepera2, Bence Horváth1, Pál Ormos1, Roberto Osellame2 and Rebeca Martínez Vázquez2
1 Biological Research Centre, Institute of Biophysics, Hungarian Academy of Sciences, Temesvári krt. 62, 6726 Szeged, Hungary;
2 Institute for Photonics and Nanotechnologies, National Research Council, Piazza Leonardo da Vinci, 32, 20133 Milan, Italy
Whispering gallery mode (WGM) resonators are promising optical structures for microfluidic label-free biosensors mainly due to their high sensitivity. Their real laboratory diagnostic application however lacks a robust fabrication method that offers a complete device of practical value. Here we report on a monolithic lab on a chip sensor fabricated by a hybrid femtosecond laser micromachining approach, for label-free biosensing of a selected protein. It consists of a polymer WGM microresonator sensor made by two-photon polymerization directly inside a glass microfludic chip prepared by laser-assisted etching. The device, after a thorough geometry optimization, presents a refractive index change sensitivity of 61 nm/RIU. We demonstrate its bio-sensing capability exploiting the biotin-streptavidin binding affinity, obtaining a detectable minimum protein surface density increase of 67 x 103 molecules/µm2.
Acknowledgements
This work was supported by funding received from the CONCERT-Japan Photonic Manufacturing Joint Call (FEASIBLE project), GINOP-2.3.2-15- 2016-00001, GINOP-2.3.3-15-2016-00040 and the European Union's Horizon 2020 Research and Innovation Programme under grant agreement No. 654148 Laserlab-Europe.